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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Neuronal cell culture in vitro
 Kohoutová, Šárka ; Bařinka, Cyril (advisor) ; Pavlíček, Jiří (referee)
Neuronal cell cultures are in vitro cultures of dissociated neurons that have become an essential part of many neurobiological experiments in the last century. Cultured neurons not only allow to answer questions about their physiology under complex in vivo conditions, but also can serve as a model of neurodegenerative diseases. Neuronal cells can either be isolated directly from the nervous tissue of animals at the prenatal or adult stage of development, or they can be obtained through targeted manipulations of stem cells and secondary cell lines that lead to their neuronal differentiation. Primary neurons are considered the gold standard of neurobiological research not only because of their long tradition of cultivation, but also because primary neurons retain typical neuronal properties under in vivo conditions. There are several disadvantages associated with primary neurons, including the fact that fully differentiated neurons do not proliferate and are relatively demanding in terms of culture conditions For this reason, their role is often replaced by mitotically active secondary cell lines or stem cells. This bachelor thesis summarizes the knowledge about cell cultures used to study the functions of neuronal cells and highlights the advantages and disadvantages of their use. Key words Primary...
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Fluidics: what it is, where it is heading - and how it will change the world we line in
 Tesař, Václav
Fluidics, technique of handling fluids, is usually meant to imply absence of moving components. It is less known and used than it deserves considering the advantages it offers. Recently its applications became more widespread thanks to two development directions. On one hand, it is the power fluidics, handling larger flow rates. Newly developed are no-moving-part fluidic pumps for dangerous liquids (in particular radioactive ones) — and fluidic oscillators for generation of microbubbles used in waste water processing and growth of unicellular plants. The other rapidly growing development direction is microfluidics, often integral with electronic circuitry on the same substrate. It has immense possibilities in sensors and also in handling small amounts of fluids e.g. in microchemistry.
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